Composite

Part:BBa_K676010:Design

Designed by: Kheng Tee Ng   Group: iGEM11_UCL_London   (2011-09-17)


GyrB cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 654
    Illegal BglII site found at 1893
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 326
    Illegal AgeI site found at 464
    Illegal AgeI site found at 1157
    Illegal AgeI site found at 2273
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GyrB has a EcoR1 restriction site at 6th bp of the ORF and must be removed either by site directed mutagenesis or artificial gene synthesis.


Source

E.coli genome

References